製品&受託サービス - MACS 細胞分離 - 自動磁気細胞分離装置 - アプリケーション

アプリケーション

autoMACS® Pro Separator は、どんな分野のどんな研究者にとっても汎用的なツールです。様々な分野の最新の学術論文や参考資料をご覧ください。

下記アプリケーションノート等をダウンロードできます:
MACS® Technology for PCR-based chimerism analysis
MACS® Technology: the complete alternative to flow sorting
autoMACS® Pro Separator in multiple myeloma research

免疫学分野

ミルテニーバイオテク社は、20年以上にわたり、免疫学分野に高品質な細胞分離ツールを提供してきました。MACS Techonology なら、機能を保持した細胞が分離でき、次の実験にもそのまま使用できます。免疫学分野のための多彩な試薬をご利用ください。

Perez, J. et al. (2011) Accumulation of CD4+ T cells in the colon of CsA-treated mice following myeloablative conditioning and bone marrow transplantation. Am. J. Physiol. Gastrointest. Liver Physiol. 300: G843–G852.

Bonnefoy, F. et al. (2011) TGF-β–exposed plasmacytoid dendritic cells participate in Th17 commitment. J. Immunol.186: 6157–6164.

Soroosh, P. et al. (2011) Herpesvirus entry mediator (TNFRSF14) regulates the persistence of T helper memory cell populations. J. Exp. Med. 208: 797–809.

Li, L. et al. (2011) Transcriptional regulation of the Th17 immune response by IKKα. J. Exp. Med. 208: 787–796.

Nanua S. et al. (2011) Activation of the unfolded protein response is associated with impaired granulopoiesis in transgenic mice expressing mutant Elane. Blood: 117: 3539–3547.

Sun J.C. et al. (2011) Homeostatic proliferation generates long-lived natural killer cells that respond against viral infection. J. Exp. Med. 208: 357–368.

Liu X. et al. (2011) High-dose dexamethasone shifts the balance of stimulatory and inhibitory Fc receptors on monocytes in patients with primary immune thrombocytopenia. Blood 117: 2061–2069.

Janikashvili N. et al. (2011) Allogeneic effector/memory Th-1 cells impair FoxP3+ regulatory T lymphocytes and synergize with chaperone-rich cell lysate vaccine to treat leukaemia. Blood 117: 1555–1564.

Sanz E. et al. (2010) Ordering human CD34+CD10–CD19+ pre/pro-B-cell and CD19– common lymphoid progenitor stages in two pro-B-cell development pathways. Proc. Natl. Acad. Sci. USA 107: 5925–5930.

Cai S.F. et al. (2010) Granzyme B is not required for regulatory T cell–mediated suppression of graft-versus-host disease. Blood 115: 1669–1677

癌研究分野

多様な癌種や、癌が進行する際に起こる細胞の変化を解析するために、癌研究者はしばしば高純度の癌細胞を必要とします。ここでは、autoMACS Pro Separator を用いた、迅速かつ簡単な高純度の癌細胞の分離をご紹介しています。

Liu, Y. et al. (2011) Expression of p16INK4a prevents cancer and promotes aging in lymphocytes. Blood 117: 3257–3267.

Vargova, K. et al. (2011) MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia. Blood 117: 3816–3825.

Tawara, I. et al. (2011) Interleukin-6 modulates graft-versus-host responses after experimental allogeneic bone marrow transplantation. Clin. Cancer Res. 17: 77–88.

Tokunaga, M. et al. (2010) BCR-ABL but not JAK2 V617F inhibits erythropoiesis through the ras signal by inducing p21CIP1/WAF1. J. Biol. Chem. 285: 31774–31782.

Thompson, E.D. et al. (2010) Tumor masses support naive T cell infiltration, activation, and differentiation into effectors. J. Exp. Med. 207: 1791–1804

幹細胞研究分野

幹細胞研究のための、autoMACS Pro Separator を用いた最新の技術をご紹介しています。

Carlson, S. et al. (2011) Cardiac mesenchymal stem cells contribute to scar formation after myocardial infarction. Cardiovasc. Res. 91: 99–107.

Chen, T. H.-P. et al. (2011) Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530–1539

Gieseke, F. et al. (2010) Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells. Blood 116: 3770–3779.

Shono, Y. et al. (2010) Bone marrow graft-versus-host disease: early destruction of hematopoietic niche after MHC-mismatched hematopoietic stem cell transplantation. Blood 115: 5401–5411.

Yamashita, T. et al. (2010) Oncostatin M renders epithelial cell adhesion molecule–positive liver cancer stem cells sensitive to 5-fluorouracil by inducing hepatocytic differentiation. Cancer Res. 70: 4687–4697.

Song, J. et al. (2010) An in vivo model to study and manipulate the hematopoietic stem cell niche. Blood 115: 2592–2600.

Gutman, J.A. et al. (2010) Single-unit dominance after double-unit umbilical cord blood transplantation coincides with a specific CD8+ T cell response against the non-engrafted unit. Blood 115: 757–765.

細胞&分子生物学分野

最高の結果は高品質なサンプルから得られます。autoMACS Pro Separator を用いれば、高感度な分子生物学的解析に必要とされる高純度かつ高生存率の細胞を得ることができます。

Zhao, L. et al. (2011) Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb. Nucleic Acids Res. 39: 4664–4679.

Tandon, P. et al. (2011) Requirement for ribosomal protein S6 kinase 1 to mediate glycolysis and apoptosis resistance induced by Pten deficiency. Proc. Natl. Acad. Sci. USA 108: 2361–2365.

Belzile, J-P. et al. (2010) HIV-1 Vpr induces the K48-linked polyubiquitination and proteasomal degradation of target cellular proteins to activate ATR andpPromote G2 arrest. J. Virol. 84: 3320 – 3330.

Jeong, S.M. et al. (2010) The SWI/SNF chromatin-remodeling complex modulates peripheral T cell activation and proliferation by controlling AP-1 expression. J. Biol. Chem. 285: 2340–2350.

神経科学分野

近年の神経科学の発展は、脳の様々な機能的な細胞の解析によってもたらされました。ミエリンの除去から特異的な神経細胞サブセットの分離まで、最高の結果のためにMACS® Technology をご利用ください。  
 
Fadul. C.E. et al. (2011) Immune modulation effects of concomitant temozolomide and radiation therapy on peripheral blood mononuclear cells in patients with glioblastoma multiforme. Neuro. Oncol. 13: 393–400.

Ferrandi, C. et al. (2011) Characterization of immune cell subsets during the active phase of multiple sclerosis reveals disease and c-Jun N-terminal kinase pathway biomarkers. Mult. Scler. 17: 43–56.

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