アプリケーションとプロトコール

簡単なものから複雑なものまで、あなたのフローサイトメトリー解析をお助けいたします。以下のアプリケーションについてはそれぞれのリンクをご覧ください。
  • 希少細胞解析
  • 細胞周期、アポトーシス解析
  • フェノタイピング
  • 腫瘍からのCSCとTILの同定と解析
  • 蛍光タンパク質解析
  • microvessicles の同定と解析

フローサイトメトリープロトコール

自動コンペンセーション
Instrument compensation is crucial for the optimal acquisition and display of data obtained by flow cytometry. Compensation essentially accounts for the inherent overlap in emission spectra observed ...
自動サンプル処理
Many flow cytometry applications require a high sample throughput and a high level of standardization. The
MACSQuant® Analyzer meets these requirements offering the capability of processing 96 samples in one go with fully automated labeling and data acquisition ...
Express Modes
The MACSQuantify™ Software includes a number of data acquisition and analysis tools that have been developed for an optimized and automated measurement of cells ...
MACSPlex Assay 他社製フローサイトメーターでのデータ解析法
MACSPlex Assays are designed for determining concentrations of soluble analytes in a single sample. A prerequisite for the performance of MACSPlex Assays is a flow cytometer with ...
溶血をしない全血の解析
For routine flow cytometric monitoring of cell populations within whole blood, there are several critical points to be considered for processing the sample material. The procedure should be ...
細胞のviability測定
Flow cytometry is a rapid and reliable method to quantify viable cells in a cell suspension. Determination of cell viability is critical when evaluating....

免疫学

7-color フェノタイピング
Flow cytometric immunophenotyping and enumeration of a comprehensive range of immune cell subsets can be a tedious challenge. Establishing an appropriate assay usually requires...
洗浄ステップ不要な全血中白血球のフローサイトメトリー解析
Many diseases can affect numbers of white blood cells (leukocytes) in peripheral blood and several assays are available to determine total leukocyte numbers. However, it is far less easy to reliably ...
 
蛍光タンパク質レポーターとマルチカラーフローサイトメトリー法を使用した造血細胞分化の解析
Hematopoietic stem and progenitor cells have the ability to differentiate into different types of mature blood cells. The process by which stem cells choose between different ...
 
IL-17-分泌細胞の解析
Interleukin 17 (IL-17, IL-17A, CTLA8), a member of the IL-17 family (IL-17A–F), is a disulfide-linked homodimeric glycoprotein. Human IL-17 consists of ...
MACSQuant Analyzerを用いた希少細胞磁気濃縮による高感度検出とフローサイトメトリー解析
The detection of rare cell types in standard flow cytometric assays is restricted by the total number of cells acquired during a single measurement. Pre-enrichment of a rare target population dramatically ...
ヒスタミンH4レセプターによるアレルギー性気管支炎の調節:フローサイトメトリー法による解析
The incidence of allergic airway diseases, such as asthma, is increasing throughout the world. Although histamine (HA) is found at high concentrations in ...
慢性リンパ性白血病の研究のためのROR1+ B細胞の高感度解析と分離
Flow cytometric analysis of lymphoid cells from chronic lymphocytic leukemia (CLL) samples requires a complex panel of multiple markers. Most of these markers are expressed on ...

ガン研究

メラノーマのTILとガン幹細胞の同定と解析
Many of the different cell types found in melanomas are of paramount interest for research into tumor biology and ultimately the development of potential treatment options. Adoptive cell therapy with tumor-infiltrating lymphocytes (TIL) ...
MACSQuant Analyzerを用いた希少細胞磁気濃縮による高感度検出とフローサイトメトリー解析
The detection of rare cell types in standard flow cytometric assays is restricted by the total number of cells acquired during a single measurement. Pre-enrichment of a rare target population dramatically increases the sensitivity ..

神経科学

ミエリン片測定のためのゲーティングストラテジー
General instructions for data acquisition and analysis of myelin debris.

幹細胞研究

蛍光タンパク質レポーターとマルチカラーフローサイトメトリー法を使用した造血細胞分化の解析
Hematopoietic stem and progenitor cells have the ability to differentiate into different types of mature blood cells. The process by which stem cells choose between different ...
CD34/CD133陽性細胞の解析
The CD34 antigen is a single-chain transmembrane glycoprotein, expressed on human hematopoietic stem
and progenitor cells constituting a small subpopulation of bone marrow cells and peripheral blood cells...

血管生物学

循環血管内皮細胞(CEC)の解析
Circulating endothelial cells (cEC) are found in human peripheral blood and have been demonstrated to increase in number when vascular damage occurs in conjunction with ...
内皮前駆細胞の解析
Experimental and clinical observations have suggested that progenitor cells in the bone marrow are mobilized and involved in various tissue regeneration processes. These stem or progenitor cells are activated and migrate ...

細胞分子生物学

蛍光タンパク質の解析
The use of flow cytometric techniques for the analysis of cell populations enables scientists to evaluate cells based on both intrinsic and extrinsic properties. Using fluorescent markers such as dyes, antibodies, or fluorescent reporters multiple characteristics of thousands of cells can be ...
細胞周期の解析
Cell proliferation is characterized by four distinct phases: G1 phase (Gap1), S phase (DNA synthesis), G2 phase (Gap2) and M phase (mitosis). Non-proliferative or “resting cells” are referred to as ...
植物細胞の倍数性レベルの解析
Misdivisions during meiosis or mitosis can lead to a multiplication of the entire set of chromosomes.
This phenomenon is called pluriploidy and occurs in many plants, in particular ...

その他のアプリケーション

microvesiclesの解析
Microvesicles (MVs) are small (0.1–1 μm) membrane vesicles shed from activated cells, delimited by a lipid bilayer and containing a wide range of ...
自社および他社製抗体の比較:MFIとSIの測定
Flow cytometry is a powerful technique to identify, enumerate, and characterize cells by immunophenotyping. However, data quality and reliability of the results critically depend on ...
MACSQuant Analyzerを用いたCHO-DG44細胞の抗体産生能、細胞密度および生存率の解析
Expression of recombinant antibodies in CHO cells is a state-of-the-art procedure in research and industry. Generation of cell lines producing high amounts of antibodies is one of the ...
MACSQuant Analyzerを用いたヒト赤血球内のマラリア原虫の各生活環ステージの解析
Plasmodium falciparum is the most deadly of the four Plasmodium species that can cause malaria and is responsible for almost a million deaths every year. P. falciparum is an ...

Contact

Miltenyi Biotec K.K.
TEL +81 3 5646 8910
FAX+81 3 5646 8911
macs@miltenyibiotec.jp
お問い合わせ

Product finder

Antibody panel builder

Toolbox

Loading...
MACSQuant Xの発売を記念してフローサイトメーターを50%OFFでご提供いたします

フローサイトメーターが50%OFF

MACSQuant Xの発売を記念して、フローサイトメーター全機種が最大50%OFF

フローサイトメトリー用抗体のチューブと濃度がより使いやすくなります

抗体フォーマット変更

フローサイトメトリー用抗体のチューブと濃度がより使いやすくなります

Change country

Please select your country to continue