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Flow cytometry has been an essential tool for immunologists over the years, as it has provided a way to identify and characterize the different cells that comprise blood. Flow cytometry is the technique of choice to identify specific immune cells, such as T cells, B cells, Monocytes, Granulocytes, natural killer (NK) cells, dendritic cells (DCs) and many more.
The MACSQuant Analyzer assays for Immunology include:
- Identification and characterization of immune cells
- Analysis of antigen-specific T cells
- Analysis of cytokine-secreting cells
- Whole blood cell analysis
- Apoptosis detection
- Proliferation assay
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References
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| Corrotte, M et al. Ral isoforms are implicated in FcgR-mediated phagocytosis: Activation of phospholipase D by RalA (2010) J Immunol. |
| Dietz, L et al. Tracking human contact allergens: From mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T cell priming (2010) Toxicol Sci. |
| Everett, H et al. Characterisation of experimental infections of domestic pigs with genotype 2.1 and 3.3 isolates of classical swine fever virus (2009) Vet. Microbiol. |
| Feng, N et al.Lineage divergence at the first TCR-dependent checkpoint: preferential gd and impaired ab T cell development in nonobese diabetic mice (2011) J Immunol. |
| Gibbings, S et al.Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, PPARg sumoylation, and HIF-1a (2010) J Biol Chem. |
| Gouin, E et al.The Listeria monocytogenese InlC protein interferes with innate immune responses by targeting the IkB kinase subunit IKKa (2010) PNAS. |
| Graham, SP et al.Characterisation of virus-specific peripheral blood cell cytokine responses following vaccination or infection with classical swine fever viruses (2010) Vet. Microbiol. |
| Gudmundsdottir, HS et al. Construction and characterization of an infectious molecular clone of maedi-visna virus that expresses green fluorescent protein (2010) J Virol Meth |
| Ishida, W et al. Adjuvants determine the contribution of basophils to antigen sensitization in vivo (2010) Immunol. Let. |
| Jun, DH et al.The pathology of bleomycin induced fibrosis is associated with loss of resident lung mesenchymal stem cells which regulate effector T cell proliferation (2011) Stem Cells. |
| Koscielny, A et al. Isolation of T cells and dendritic cells from peripheral intestinal tissue, Peyer’s Patches and mesenteric lymph nodes in mice after intestinal manipulation (2011) Protocol Exchange. |
| Lexberg, MH et al. IFN-g and IL-12 synergize to convert in vivo-generated Th17 into Th1/17 cells (2010) Eur J Immunol. |
| McKimmie, C et al. A TLR2 ligand suppresses inflammation by modulation of chemokine receptors and redirection of leukocyte migration (2009) Blood. |
| Najar, M et al. Mesenchymal stromal cells promote or suppress the proliferation of T lymphocytes from cord blood and peripheral blood: the importance of low cell ratio and role of interleukin-6 (2009) Cytotherapy. |
| Petzke, MM et al.Recognition of Borrelia burgdorferi, the Lyme Disease Spirochete, by TLR7 and TLR9 Induces a Type I IFN Response by Human Immune Cells (2009) J Immunol. |
| Saha, S et al.Peptidoglycan recognition proteins protect mice from experimental colitis by promoting normal gut flora and preventing induction of Interferon-g. (2010) Cell Host & Microbe. |
| Stamatopoulous, B et al.The histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) induces apoptosis, downregulated the CXCR4 chemokine receptor and impairs migration of chronic lymphocytic leukemia cells (2010) Haematologica. |
| Tourret, M et al. T cell polarity at the immunological synapse is required for CD154-dependent IL-12 secretion by dendritic cells (2010) J Immunol. |
| West, JA et al.Activation of Plasmacytoid Dendritic Cells by KSHV (2011) J Virol. |
| Zhang, W et al. PD-L1 blockade improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction (2010) Critical Care. |
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