CD134 (OX40) 抗体 (ヒト)
Overview この抗体は、抗原特異的CD4＋T細胞の検出など2 、フローサイトメトリーや蛍光顕微鏡法によるCD134 (OX40)+ヒト細胞の同定や解析に適しています。 Details Background information クローンACT35は、腫瘍壊死因子/神経成長因子レセプター(TNFR/NGFR)ファミリーの一員であるCD134 (OX40)抗原を認識します。CD134は、50 kDaのタイプⅠ膜型糖タンパク質で、活性化Tリンパ球に発現しています。OX40LとCD134の相互作用は、T細胞依存的な体液性免疫反応、プライマリーT細胞のexpansionの調節、T細胞の生存、メモリーT細胞プールのサイズ、CD4+ T細胞トレランスの調節に関与しています1。 Clone Isotype ACT35 Mouse IgG1 Further information Enrichment of CD134 (OX40)+ cells [PDF; 265,1 KB]		Figure 1 Human peripheral blood mononuclear cells (PBMCs) were stimulated (A) with CytoStim (1:50, 14 h) or left untreated (B) and stained with CD134 (OX40) antibodies conjugated to PE as well as with CD4 (VIT4)-FITC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. A: Stimulated sample B: Unstimulated control Figure 2 Human peripheral blood mononuclear cells (PBMCs) were stimulated (A) with CytoStim (1:50, 14 h) or left untreated (B) and stained with CD134 (OX40) antibodies conjugated to APC as well as with CD4 (VIT4)-FITC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. A: Stimulated sample B: Unstimulated control Figure 3 Human peripheral blood mononuclear cells (PBMCs) were stimulated (A) with CytoStim (1:50, 14 h) or left untreated (B) and stained with CD134 (OX40)-Biotin, Anti-Biotin-APC and CD4 (VIT4)-FITC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. A: Stimulated sample B: Unstimulated control

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