MACS® Neuro Medium
Overview MACS Neuro Medium は、マウス、ラットならびにヒトの中枢神経系 （CNS）や末梢神経系 (PNS)の細胞増殖や長期培養生存に最適化された無血清培地です¹,²。 Details MACS Neuro Medium は、Neural Tissue Dissociation Kits による組織分散後や細胞分離後の神経系細胞の培養に適しています。MACS Neuro Medium に適切なグロースファクターやサプリメントを添加することで、磁気分離後の神経幹・前駆細胞よりニューロスフェアの形成 (neurosphere assay) が可能です。さらに、生後から成体までの様々な組織から磁気分離した神経系の細胞、分化した細胞あるいはニューロン前駆細胞、グリア前駆細胞の培養が可能です。CNSおよびPNSの神経系細胞の増殖ならびに長期培養生存、低密度培養には、MACS Supplement B27 PLUS をお勧めします。 Further information Neuroscience research brochure [PDF; 1,7 MB]		Figure 1 Light microscopic picture of neurosphere formation after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (P) and magnetically isolated using Anti-Prominin-1 MicroBeads prior to cell culture. Figure 2 Light microscopic picture of PSA-NCAM–positive neuronal precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T). Figure 3 Light microscopic picture of A2B5-positive glial precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. A2B5-positive cells were processed for immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a marker for glial precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

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