Pluripotent Stem Cell マイクロビーズ
Description Pluripotent Stem Cell MicroBeads は、CD326 抗原 (EpCAM) の発現に基づいて ヒト多能性幹細胞を分離するために開発されました。CD326 は、未分化なヒト胚性幹細胞 （ES細胞）およびヒト人工多能性幹細胞 (iPS細胞) で発現しており、OCT4 陽性多能性細胞に局在しています。 また、その発現は、分化が始まるとダウンレギュレートされます¹,²。 Applications ヒトES / iPS 細胞等の未分化なCD326+ 多能性幹細胞のポジティブセレクション リプログラミング後のiPS細胞の濃縮 Columns LS 又は autoMACS カラム Further information Stem Cell Research Brochure [PDF; 3,8 MB] Stem Cell Product List Sept 2011 [PDF; 709,8 KB]		Pluripotent CD326+ iPS cells were isolated from cultures containing spontaneously differentiated iPS cells grown on mouse embryonic feeder cells using Pluripotent Stem Cell MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD326 (EpCAM)-APC, Stemgent® Phycoerythrin (PE) Anti-Human TRA-1-60 Antibody (# 130-095- 624), and Anti-Feeder-Biotin (coming soon) as well as with Anti- Biotin-FITC, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Feeder cells were excluded based on staining with Anti-Feeder-Biotin.

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